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rat anti mouse upa  (R&D Systems)


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    Structured Review

    R&D Systems rat anti mouse upa
    Rat Anti Mouse Upa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+anti+mouse+upa/Mouse+u-Plasminogen+Activator+(uPA)%2FUrokinase+Antibody/pmc12839967-265-42-47
    Average 94 stars, based on 2 article reviews
    rat anti mouse upa - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Single Cell:

    Article Title: uPA-mediated remodeling of CCL21 gradients regulates lymphatic migration of dendritic cells
    Article Snippet: The tissue pieces were digested in 4 mg/ml collagenase IV (Thermo Fisher Scientific) in PBS at 37°C for 45 min, passed through a 40-μm cell strainer, and washed with FACS buffer (PBS containing 1% FBS). .. Single-cell suspensions were stained on ice in FACS buffer according to the following four-step protocol (for 20 min each step, all antibodies from BioLegend unless indicated otherwise): (1) anti-mouse CD16/32 (Fc Block) (clone 93); (2) goat anti-mouse uPAR (polyclonal, AF534; R&D Systems), rat anti-mouse uPA (Clone 901420; R&D Systems), and goat anti-mouse kringle-5 plasminogen (polyclonal, AF742; R&D Systems); (3) donkey anti-rabbit/goat/rat Alexa 488/594/647 (all Invitrogen). (4) Rat anti-mouse CD31 BV421 (clone MEC 13.3; BD Pharmingen), rat anti-mouse CD45 APC/Cy7 (clone 30-F11; BioLegend), Syrian hamster anti-mouse Podoplanin PE/Cy7 (clone 8.1.1; BioLegend), and Zombie Aqua. ..

    Article Title: uPA-mediated remodeling of CCL21 gradients regulates lymphatic migration of dendritic cells.
    Article Snippet: The tissue pieces were digested in 4 mg/ml collagenase IV (Thermo Fisher Scientific) in PBS at 37°C for 45 min, passed through a 40-μm cell strainer, and washed with FACS buffer (PBS containing 1% FBS). .. Single-cell suspensions were stained on ice in FACS buffer according to the following four-step protocol (for 20 min each step, all antibodies from BioLegend unless indicated otherwise): (1) anti-mouse CD16/32 (Fc Block) (clone 93); (2) goat anti-mouse uPAR (polyclonal, AF534; R&D Systems), rat anti-mouse uPA (Clone 901420; R&D Systems), and goat anti-mouse kringle-5 plasminogen (polyclonal, AF742; R&D Systems); (3) donkey anti-rabbit/goat/ rat Alexa 488/594/647 (all Invitrogen). (4) Rat anti-mouse CD31 BV421 (clone MEC 13.3; BD Pharmingen), rat anti-mouse CD45 APC/Cy7 (clone 30-F11; BioLegend), Syrian hamster anti-mouse Podoplanin PE/Cy7 (clone 8.1.1; BioLegend), and Zombie Aqua. ..

    Staining:

    Article Title: uPA-mediated remodeling of CCL21 gradients regulates lymphatic migration of dendritic cells
    Article Snippet: The tissue pieces were digested in 4 mg/ml collagenase IV (Thermo Fisher Scientific) in PBS at 37°C for 45 min, passed through a 40-μm cell strainer, and washed with FACS buffer (PBS containing 1% FBS). .. Single-cell suspensions were stained on ice in FACS buffer according to the following four-step protocol (for 20 min each step, all antibodies from BioLegend unless indicated otherwise): (1) anti-mouse CD16/32 (Fc Block) (clone 93); (2) goat anti-mouse uPAR (polyclonal, AF534; R&D Systems), rat anti-mouse uPA (Clone 901420; R&D Systems), and goat anti-mouse kringle-5 plasminogen (polyclonal, AF742; R&D Systems); (3) donkey anti-rabbit/goat/rat Alexa 488/594/647 (all Invitrogen). (4) Rat anti-mouse CD31 BV421 (clone MEC 13.3; BD Pharmingen), rat anti-mouse CD45 APC/Cy7 (clone 30-F11; BioLegend), Syrian hamster anti-mouse Podoplanin PE/Cy7 (clone 8.1.1; BioLegend), and Zombie Aqua. ..

    Article Title: uPA-mediated remodeling of CCL21 gradients regulates lymphatic migration of dendritic cells.
    Article Snippet: The tissue pieces were digested in 4 mg/ml collagenase IV (Thermo Fisher Scientific) in PBS at 37°C for 45 min, passed through a 40-μm cell strainer, and washed with FACS buffer (PBS containing 1% FBS). .. Single-cell suspensions were stained on ice in FACS buffer according to the following four-step protocol (for 20 min each step, all antibodies from BioLegend unless indicated otherwise): (1) anti-mouse CD16/32 (Fc Block) (clone 93); (2) goat anti-mouse uPAR (polyclonal, AF534; R&D Systems), rat anti-mouse uPA (Clone 901420; R&D Systems), and goat anti-mouse kringle-5 plasminogen (polyclonal, AF742; R&D Systems); (3) donkey anti-rabbit/goat/ rat Alexa 488/594/647 (all Invitrogen). (4) Rat anti-mouse CD31 BV421 (clone MEC 13.3; BD Pharmingen), rat anti-mouse CD45 APC/Cy7 (clone 30-F11; BioLegend), Syrian hamster anti-mouse Podoplanin PE/Cy7 (clone 8.1.1; BioLegend), and Zombie Aqua. ..

    FACS:

    Article Title: uPA-mediated remodeling of CCL21 gradients regulates lymphatic migration of dendritic cells
    Article Snippet: The tissue pieces were digested in 4 mg/ml collagenase IV (Thermo Fisher Scientific) in PBS at 37°C for 45 min, passed through a 40-μm cell strainer, and washed with FACS buffer (PBS containing 1% FBS). .. Single-cell suspensions were stained on ice in FACS buffer according to the following four-step protocol (for 20 min each step, all antibodies from BioLegend unless indicated otherwise): (1) anti-mouse CD16/32 (Fc Block) (clone 93); (2) goat anti-mouse uPAR (polyclonal, AF534; R&D Systems), rat anti-mouse uPA (Clone 901420; R&D Systems), and goat anti-mouse kringle-5 plasminogen (polyclonal, AF742; R&D Systems); (3) donkey anti-rabbit/goat/rat Alexa 488/594/647 (all Invitrogen). (4) Rat anti-mouse CD31 BV421 (clone MEC 13.3; BD Pharmingen), rat anti-mouse CD45 APC/Cy7 (clone 30-F11; BioLegend), Syrian hamster anti-mouse Podoplanin PE/Cy7 (clone 8.1.1; BioLegend), and Zombie Aqua. ..

    Article Title: uPA-mediated remodeling of CCL21 gradients regulates lymphatic migration of dendritic cells.
    Article Snippet: The tissue pieces were digested in 4 mg/ml collagenase IV (Thermo Fisher Scientific) in PBS at 37°C for 45 min, passed through a 40-μm cell strainer, and washed with FACS buffer (PBS containing 1% FBS). .. Single-cell suspensions were stained on ice in FACS buffer according to the following four-step protocol (for 20 min each step, all antibodies from BioLegend unless indicated otherwise): (1) anti-mouse CD16/32 (Fc Block) (clone 93); (2) goat anti-mouse uPAR (polyclonal, AF534; R&D Systems), rat anti-mouse uPA (Clone 901420; R&D Systems), and goat anti-mouse kringle-5 plasminogen (polyclonal, AF742; R&D Systems); (3) donkey anti-rabbit/goat/ rat Alexa 488/594/647 (all Invitrogen). (4) Rat anti-mouse CD31 BV421 (clone MEC 13.3; BD Pharmingen), rat anti-mouse CD45 APC/Cy7 (clone 30-F11; BioLegend), Syrian hamster anti-mouse Podoplanin PE/Cy7 (clone 8.1.1; BioLegend), and Zombie Aqua. ..

    Blocking Assay:

    Article Title: uPA-mediated remodeling of CCL21 gradients regulates lymphatic migration of dendritic cells
    Article Snippet: The tissue pieces were digested in 4 mg/ml collagenase IV (Thermo Fisher Scientific) in PBS at 37°C for 45 min, passed through a 40-μm cell strainer, and washed with FACS buffer (PBS containing 1% FBS). .. Single-cell suspensions were stained on ice in FACS buffer according to the following four-step protocol (for 20 min each step, all antibodies from BioLegend unless indicated otherwise): (1) anti-mouse CD16/32 (Fc Block) (clone 93); (2) goat anti-mouse uPAR (polyclonal, AF534; R&D Systems), rat anti-mouse uPA (Clone 901420; R&D Systems), and goat anti-mouse kringle-5 plasminogen (polyclonal, AF742; R&D Systems); (3) donkey anti-rabbit/goat/rat Alexa 488/594/647 (all Invitrogen). (4) Rat anti-mouse CD31 BV421 (clone MEC 13.3; BD Pharmingen), rat anti-mouse CD45 APC/Cy7 (clone 30-F11; BioLegend), Syrian hamster anti-mouse Podoplanin PE/Cy7 (clone 8.1.1; BioLegend), and Zombie Aqua. ..

    Article Title: uPA-mediated remodeling of CCL21 gradients regulates lymphatic migration of dendritic cells.
    Article Snippet: The tissue pieces were digested in 4 mg/ml collagenase IV (Thermo Fisher Scientific) in PBS at 37°C for 45 min, passed through a 40-μm cell strainer, and washed with FACS buffer (PBS containing 1% FBS). .. Single-cell suspensions were stained on ice in FACS buffer according to the following four-step protocol (for 20 min each step, all antibodies from BioLegend unless indicated otherwise): (1) anti-mouse CD16/32 (Fc Block) (clone 93); (2) goat anti-mouse uPAR (polyclonal, AF534; R&D Systems), rat anti-mouse uPA (Clone 901420; R&D Systems), and goat anti-mouse kringle-5 plasminogen (polyclonal, AF742; R&D Systems); (3) donkey anti-rabbit/goat/ rat Alexa 488/594/647 (all Invitrogen). (4) Rat anti-mouse CD31 BV421 (clone MEC 13.3; BD Pharmingen), rat anti-mouse CD45 APC/Cy7 (clone 30-F11; BioLegend), Syrian hamster anti-mouse Podoplanin PE/Cy7 (clone 8.1.1; BioLegend), and Zombie Aqua. ..



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    Expression of fibrinolytic system components in 24 h and 7 days old venous thrombi. Panels A-F show the expression (red) of (A-B) tPA, (C-D) <t>uPA,</t> (E-F) PAI-1, (G-H) plasminogen (Pg), (I-J) α2AP, and (K-L) their quantification in 8-µm cryosections of 24 hours and 7 days thrombi as labeled. Red color (Alexa Fluor 555) represents each component as labeled and blue color shows 4′,6-diamidino-2-phenylindole–stained nuclei. For comparison, IVC from sham mice were immunostained and quantified for each antibody under the same color range in the histogram mode of Image Pro-Plus software. The total immunostained area (arbitrary units; AU/mm2) for each protein was measured in 500 μm (original magnification ×4) images of an IVC sample. (N = 4-5 per group, mean ± SEM). *P < .05, **P < .01, ***P < .001.
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    Image Search Results


    Expression of fibrinolytic system components in 24 h and 7 days old venous thrombi. Panels A-F show the expression (red) of (A-B) tPA, (C-D) uPA, (E-F) PAI-1, (G-H) plasminogen (Pg), (I-J) α2AP, and (K-L) their quantification in 8-µm cryosections of 24 hours and 7 days thrombi as labeled. Red color (Alexa Fluor 555) represents each component as labeled and blue color shows 4′,6-diamidino-2-phenylindole–stained nuclei. For comparison, IVC from sham mice were immunostained and quantified for each antibody under the same color range in the histogram mode of Image Pro-Plus software. The total immunostained area (arbitrary units; AU/mm2) for each protein was measured in 500 μm (original magnification ×4) images of an IVC sample. (N = 4-5 per group, mean ± SEM). *P < .05, **P < .01, ***P < .001.

    Journal: Blood

    Article Title: Venous stasis-induced fibrinolysis prevents thrombosis in mice: role of α2-antiplasmin

    doi: 10.1182/blood.2019000049

    Figure Lengend Snippet: Expression of fibrinolytic system components in 24 h and 7 days old venous thrombi. Panels A-F show the expression (red) of (A-B) tPA, (C-D) uPA, (E-F) PAI-1, (G-H) plasminogen (Pg), (I-J) α2AP, and (K-L) their quantification in 8-µm cryosections of 24 hours and 7 days thrombi as labeled. Red color (Alexa Fluor 555) represents each component as labeled and blue color shows 4′,6-diamidino-2-phenylindole–stained nuclei. For comparison, IVC from sham mice were immunostained and quantified for each antibody under the same color range in the histogram mode of Image Pro-Plus software. The total immunostained area (arbitrary units; AU/mm2) for each protein was measured in 500 μm (original magnification ×4) images of an IVC sample. (N = 4-5 per group, mean ± SEM). *P < .05, **P < .01, ***P < .001.

    Article Snippet: The primary antibodies include rabbit anti-mouse against tPA (ASMTPA-GF; Molecular Innovations, Novi, MI), uPA (ASMUPA-GF-HT; Molecular Innovations, Novi, MI), PAI-1 (IASMPAI-GF; Innovative Research, Novi, MI), plasminogen (Abcam; Cambridge, MA); goat anti-mouse α2AP (AF1239; R&D Systems, Minneapolis, MN); rat anti-mouse Ly6G (clone1A8, #127602; BioLegend, San Diego, CA), and Ly6B.2 (#771G; Bio-Rad Laboratories, Hercules, CA).

    Techniques: Expressing, Labeling, Staining, Software